Composite

Part:BBa_K3002227:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 hygromycin resistance + ccA_Mut-PETase_SP20HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1789
    Illegal PstI site found at 2873
    Illegal PstI site found at 3846
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1789
    Illegal NheI site found at 2053
    Illegal PstI site found at 2873
    Illegal PstI site found at 3846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1789
    Illegal BamHI site found at 1408
    Illegal XhoI site found at 250
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1789
    Illegal PstI site found at 2873
    Illegal PstI site found at 3846
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1789
    Illegal PstI site found at 2873
    Illegal PstI site found at 3846
    Illegal NgoMIV site found at 773
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1326
    Illegal NgoMIV site found at 2608
    Illegal NgoMIV site found at 2635
    Illegal NgoMIV site found at 4406
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002119 (hygromycin resistance) and BBa_K3002109 (cCA_Mut-PETase_SP20HA).

References